بسم الله الرحمن الرحيم IDENTIFICATION OF FUNGI Specimen collection Specimens for fungal microscopy and culture may be: Scrapings of scale, after cleaning with alcohol. Skin stripped off with adhesive tape, which is then stuck on a glass slide. Hair which has been pulled out from the roots. Brushings from an area of scaly scalp. Specimens for fungal microscopy and culture may be: Nail clippings. Skin biopsy. Moist swab from a mucosal surface (inside the mouth or vagina) in a special transport medium. A swab should be taken from pustules in case of secondary bacterial infection. Morphology Direct Microscopy Potassium hydroxide (KOH) stained with blue or black ink. Unstained wet-mount. Stained dried smear. Histopathology of biopsy with special stains. Types of fungi A.Molds (filamentous fu
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