Quantitative Determinations of Proteins
بسم الله الرحمن الرحيم ovalbumin (Ova) or bovine serum albumin (BSA) in 0.1% SDS (w/v) is suitable. This solution can be stored in the refrigerator for several weeks. A 20g Na2CO3 (anhydrous) in 1000 ml 0.1 N NaOH Solutions/Reagents B 1.0g CuSO4 · 5H2O in 100 ml ddH2O C 2.0g potassium-sodium tartrate in 100 ml ddH2O D mix1vol. B and 1 vol. C, and then add 50 vol. A E Folin–Ciocalteu’s phenol reagent (stock), 1+1 diluted with ddH2O Standard 5.0 mg/ml ovalbumin or BSA, 0.1% SDS (w/v) in ddH2O Table 1.2. LOWRY standard protocol Blank Standard Sample (ml) – Max. 0.1 – – – Max. 0.1 H2O 0.1 to 0.1 to 0.1 Soln. D 2.0 2.0 2.0 Mix, incubate for 5–10 min at RT Soln. E 0.2 0.2 0.2 Mix, incubate for 30–45 min at RT, read at 700 nm Make samples, blank, and standards at least in duplicates, and Standard protocol measure in a spectrophotometer at 700–750 nm. Especially for sma